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Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance

Gary D. Foster
4.9/5 (15099 ratings)
Read Now Download Now ⏭ listen AudioBook
Description:A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance. To get started finding Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance, you are right to find our website which has a comprehensive collection of manuals listed.
Our library is the biggest of these that have literally hundreds of thousands of different products represented.
Pages
—
Format
PDF, EPUB & Kindle Edition
Publisher
—
Release
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ISBN
0896033856

Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance

Gary D. Foster
4.4/5 (1290744 ratings)
Read Now Download now ⏭ listen AudioBook
Description: A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance. To get started finding Methods in Molecular Biology, Volume 81: Plant Virology Protocols: From Virus Isolation to Transgenic Resistance, you are right to find our website which has a comprehensive collection of manuals listed.
Our library is the biggest of these that have literally hundreds of thousands of different products represented.
Pages
—
Format
PDF, EPUB & Kindle Edition
Publisher
—
Release
—
ISBN
0896033856

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